Thin Cell Layer Culture of <i>Chukrasia tabularis</i> A. Fuss and <i>Coffea</i> sp.

Vietnam has planning in re-forest of Chukrasia tabularis, a special-use forest and re-culture Coffea sp., an industrial crops. The first barrier is seedlings for supplying higher demand from farmers. Using thin cell layer culture method one the ways to resolve woody species. Results gave capacities using shoot-tips regeneration them far propagation. tabularis A. Juss: Shoot tip pot was used as material establish vitro plant cultured on medium MS + BA (0.1mg/l) rise up 5.7 shoots/sample shoot height 12mm after 30 days cultivation. In plantlets were materials Samples leaves slices CW (10%) initiate 2.2 shoots/leaf sample 5.8 shoots/slices sample. 2 genotypes Con Dao Lang Son show not different initiation 5.4-5.6 shoots/sample. Shoots regenerated rooted well NAA (0.1mg/l). Juss established. Coffea sp.: set plantlet by cultiavting WPM 45 days. Adenin (20mg/l) kinetin (1mg/l) (3mg/l) 5.2 (1cm2) 6.2 shoots/shoot meristem clones sp. C. robusta arabica cultivation imitiate 4-6 shoots/robusta leaf 3-5 shoots/arabica Plantlets IBA (0.6mg/l).